Journal: International Journal of Molecular Sciences
Article Title: Melatonin Modulation of Radiation and Chemotherapeutics-induced Changes on Differentiation of Breast Fibroblasts
doi: 10.3390/ijms20163935
Figure Lengend Snippet: Effects of melatonin on docetaxel- and vinorelbine-induced changes on ( A ) cell proliferation and ( B ) intracytoplasmic triglyceride content on differentiating human breast adipocytes. Breast preadipocytes completely differentiated into adipocytes were treated with docetaxel (1 µM), vinorelbine (1 µM), and/or melatonin (1 mM or 1 nM) for 3 days. ( A ) Cell proliferation was measured by the MTT method. ( B ) Intracytoplasmic triglyceride accumulation was quantified by the Oil Red O staining method. Data are expressed as the percentage of the control group (mean ± SEM). a, p < 0.001 vs. C; b, p < 0.001 vs. M (1 mM); c, p < 0.001 vs. D (1 µM); d, p < 0.001 vs. V (1 µM). C: control, M: melatonin, D: docetaxel, V: vinorelbine.
Article Snippet: Human breast preadipocytes were purchased from ZenBio, Inc (Research Triangle Park, NC, USA).
Techniques: Staining, Control